Journal: Biotechnology and Bioengineering
Article Title: Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures
doi: 10.1002/bit.25332
Figure Lengend Snippet: Selective CDK4/6 inhibition by small molecule compound. (A) Schematic diagram of the cell cycle and function of a selective CDK4/6 specific inhibitor. E2F, E2 transcription factor; DP, E2F dimerization partner; R, restriction checkpoint. (B) Whole cell extracts with or without CCI treatment were assayed for protein levels of phospho-Rb (p-Rb), phospho-ERK (p-ERK), phospho-S6 (p-S6) by Western blot. Rab11 was used as a loading control.
Article Snippet: Membrane were probed with phosphor-specific Rb (Ser 795 ), Phospho-specific ERK (T 202 /Y 204 ), Phospho-specific S6 (S 235/236 ), Rab 11 primary antibodies (Cell Signaling Technology, Denver, MA), followed by AlexaFluor® 680-conjugated secondary antibodies (Invitrogen, Carlsbad, CA).
Techniques: Inhibition, Western Blot, Control